Product Name :
ULK3 Recombinant Rabbit Monoclonal Antibody [PSH01-09]
Predicted band size :
53 kDa
Observed band size :
53 kDa
Synonyms:
DKFZP434C131 antibody FLJ90566 antibody Serine/threonine-protein kinase ULK3 antibody Ulk3 antibody ULK3_HUMAN antibody unc 51 like kinase 3 (C. elegans) antibody Unc-51-like kinase 3 antibody
Function :
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within human ULK3 aa 1-472 / 472.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF-Cell, FC, IF-Tissue
Coupling :
unconjugated
Clone number:
PSH01-09
RRID:
AB_3072734
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Formula of 333973-51-6 Aliquot store at -20℃.1239591-03-7 Formula Avoid repeated freeze / thaw cycles.PMID:33580868
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 53 kDa
Positive control :
293T cell lysate, HeLa cell lysate, Jurkat cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human testis tissue, human brain tissue, mouse brain tissue, rat brain tissue, HeLa.