Product Name :
SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99]
Predicted band size :
76 kDa
Observed band size :
100 kDa
Synonyms:
100 kDa DNA pairing protein antibody 100 kDa DNA-pairing protein antibody 100 kDa subunit antibody DNA binding p52/p100 complex 100 kDa subunit antibody DNA-binding p52/p100 complex antibody hPOMp100 antibody Polypyrimidine tract binding protein associated splicing factor antibody Polypyrimidine tract-binding protein-associated-splicing factor antibody POMP100 antibody PPP1R140 antibody 展开
Function :
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3′ side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human SFPQ aa 658-707 / 707.5-Bromo-2-methylpyridin-4-ol Chemscene
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF-Cell, IF-Tissue
Coupling :
unconjugated
Clone number:
JU33-99
RRID:
AB_3070719
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.1310481-47-0 In stock 4), 0.PMID:33471221 05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 76 kDa
Positive control :
Jurkat cell lysate, 293T cell lysate, HeLa cell lysate, K-562 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Mouse lung tissue lysate, mouse brain tissue, rat brain tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue.